Histopathological detection of hog cholera from field outbreaks

The study was carried out to detect and describe the outbreaks of Hog Cholera (HC) or Classical Swine Fever (CSF) in different parts of NorthEastern region of India by histopathological techniques in post mortem samples. Histopathological alterations in different lymphoid and non-lymphoid organs provides clue to confirm hog cholera disease. Pigs died of suspected swine fever (hog cholera) virus infection were subjected to postmortem examination and gross lesions were recorded. Samples like kidneys, mesenteric lymph node, spleen and tonsils of approximately 8x8cm size were collected and preserved in10% formal saline solution. Histopathologies were done as per the method described by Luna, 1968. From 9 outbreaks of hog cholera, 60 samples were collected for histopathological examination. All samples (Tonsil, Kidney, Mesenteric Lymph Node (MLN), spleen and brain) were found positive for hog cholera on basis of gross and microscopic findings. For detection of Hog Cholera disease, Tonsil, Kidney, Mesenteric Lymph Node (MLN), spleen and brain were found very suitable samples. Presence of pathogonomic changes in post mortal samples confirms the outbreak of Hog Cholera disease.